Serratia marcescens

Once considered a harmless saprophyte, Serratia marcescens is now recognized as an important opportunistic pathogen combining a propensity for healthcare-associated infection and antimicrobial resistance. Serratia marcescens is a member of the genus Serratia, which is a part of the family Enterobacteriaceae. Currently 14 species ofSerratia are recognized within the genus, eight of which are associated

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Actinobaculum schaalii, a Common Uropathogen in Elderly Patients, Denmark

Actinobaculum schaalii can cause urinary tract infections and septicemia but is diffi cult to identify by cultivation. To obtain a fast diagnosis and identify A. schaalii, we developed a TaqMan real-time quantitative PCR. Routine urine samples were obtained from 177 hospitalized patients and 75 outpatients in Viborg County, Denmark, in 2008–2009. The PCR detected A.

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A fatal case of urosepsis due to Corynebacterium riegelii

Corynebacterium species other than Corynebacterium diphtheriae rarely cause infections in human but rather reside in flora, however they have been reported to cause opportunistic infections in both immunocompromised and immunecompetent patients. Here we report for the first time a case of an elderly female patient presenting with a fatal urosepsis caused by a recently defined

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Urinary Tract Infections in Long-Term–Care Facilities

Urinary tract infection (UTI) is the most common bacterial infection occurring in residents of long-term–care facilities. It is a frequent reason for antimicrobial administration, but antimicrobial use for treating UTIs is often inappropriate. Achieving optimal management of UTI in this population is problematic because of the very high prevalence of bacteriuria, evidence that the treatment

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The Problem of Culture-Negative Infections

Abstract Because modern medicine suffers increasingly from the “silo” phenomenon, in which each specialty ponders its problems in isolation, the gradual emergence of a generalized threat to millions of patients is thus poorly countered by the disconnected efforts of small teams that address the same theme without the recognition of common ground. The recent recognition

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PCR Test Bests Urine Cultures for UTI Pathogen Detection

CHICAGO—A multiplex polymerase chain reaction (PCR) test identifies more bacteria than traditional urine culture in patients with symptomatic urinary tract infection (UTI), and it was able to detect more fastidious bacteria, investigators reported at the 2018 annual meeting of the Large Urology Group Practice Association. It also detected the same organisms as traditional culture. In

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Applications of Clinical Microbial Next-Generation Sequencing

The American Academy of Microbiology (Academy) is the honorific branch of the American Society for Microbiology (ASM), a nonprofit scientific society with nearly 40,000 members. Fellows of the Academy have been elected by their peers in recognition of their outstanding contributions to the microbial sciences. Through its colloquium program, the Academy draws on the expertise

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Next-Generation Sequencing of Infectious Pathogens

Next-generation sequencing holds potential for improving clinical and public health microbiology.1 In addition to identifying pathogens more rapidly and precisely than traditional methods, high-throughput technologies and bioinformatics can provide new insights into disease transmission, virulence, and antimicrobial resistance. The US public health system is integrating pathogen genome sequencing into infectious disease surveillance with support from

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Metagenomics in diagnosis and improved targeted treatment of UTI

Abstract Introduction The genomic revolution has transformed our understanding of urinary tract infection. There has been a paradigm shift from the dogmatic statement that urine is sterile in healthy people, as we are becoming forever more familiar with the knowledge that bacterial communities exist within the urinary tracts of healthy people. Metagenomics can investigate the

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A Head-to-Head Comparative Phase II Study of Standard Urine Culture and Sensitivity Versus DNA Next-generation Sequencing Testing for Urinary Tract Infections

Many studies have discussed clinical practice guidelines for the treatment of cystitis and pyelonephritis. Treatment of uncomplicated urinary tract infections (UTIs) can be based on empiric antibiotic therapy. For complicated or recurrent UTIs, therapy can be based on laboratory-controlled culture and sensitivity (C&S) reports. The diagnosis of UTI by clinical criteria alone has an error

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Evidence of Uncultivated Bacteria in the Adult Female Bladder

Clinical urine specimens are usually considered to be sterile when they do not yield uropathogens using standard clinical cultivation procedures. Our aim was to test if the adult female bladder might contain bacteria that are not identified by these routine procedures. An additional aim was to identify and recommend the appropriate urine collection method for

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Increased human pathogenic potential of Escherichia coli from polymicrobial urinary tract infections in comparison to isolates from monomicrobial culture samples

The current diagnostic standard procedure outlined by the Health Protection Agency for urinary tract infections (UTIs) in clinical laboratories does not report bacteria isolated from samples containing three or more different bacterial species. As a result many UTIs go unreported and untreated, particularly in elderly patients, where polymicrobial UTI samples are especially prevalent. This study

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A Head-to-Head Comparative Phase II Study of Standard Urine Culture and Sensitivity Versus DNA Next-generation Sequencing Testing for Urinary Tract Infections

Many studies have discussed clinical practice guidelines for the treatment of cystitis and pyelonephritis. Treatment of uncomplicated urinary tract infections (UTIs) can be based on empiric antibiotic therapy. For complicated or recurrent UTIs, therapy can be based on laboratory-controlled culture and sensitivity (C&S) reports. The diagnosis of UTI by clinical criteria alone has an error

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Next Generation Sequence for Diagnosis and Targeted Treatment

The Burden of UTIs The treatment of urinary tract infections (UTIs) is a highly demanding area for practicing urologists. UTIs carry an immense burden in both human and financial terms. One in two women will have a UTI in her lifetime, and the recurrence rate for these infections is high. The direct and indirect cost

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Asymptomatic Bacteriuria in Adults

A common dilemma in clinical medicine is whether to treat asymptomatic patients who present with bacteria in their urine. There are few scenarios in which antibiotic treatment of asymptomatic bacteruria has been shown to improve patient outcomes. Because of increasing antimicrobial resistance, it is important not to treat patients with asymptomatic bacteriuria unless there is

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